Generation of hiPSC line TCIERi001-A from normal human epidermal keratinocytes

Rupendra Shrestha¹, Yao-Tseng Wen², Rong-Kung Tsai³

Institute of Medical Sciences, Tzu Chi University, Hualien 970, Taiwan; Institute of Eye Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan.
Institute of Eye Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan.
Institute of Medical Sciences, Tzu Chi University, Hualien 970, Taiwan; Institute of Eye Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan.

Publication: Stem Cell Research. 2019 Dec:41:101590. doi: 10.1016/j.scr.2019.101590

Abstract– Human induced pluripotent stem cell (hiPSC) line TCIERi001-A was generated from normal human epidermal keratinocytes (NHEK) primary cell line with the nonintegrating system using Sendai reprogramming kit. Sendai particles were used to deliver the defined transcription factors that included three vector preparations, such as polycistronic KLF4–OCT3/4–SOX2, cMYC, and KLF4.

Cite: Shrestha R, Wen YT, Tsai RK. Generation of hiPSC line TCIERi001-A from normal human epidermal keratinocytes. Stem Cell Res. 2019 Dec;41:101590. PMID: 31683100.