Professional Experiences/Training

1.2. SickKids Hospital for Sick Children and University of Toronto, Toronto, Canada

• Fellowship Training: iPSC Workshop [7-10 May 2019]

• The Cystic Fibrosis Canada-SickKids Program for Individualized CF Therapy (CFIT)
• Directed Differentiation of iPS Cells into Systems to Model CF Lung Disease

1.3. Institute of Bioinformatics and Applied Biotechnology-IBAB, Bangalore, India.

• Department of Cell and Molecular Biology
• Research Fellow: Research Work [Jun 2015 – Jun 2016]

• Cloning, Gene Expression, Protein Expression, Protein Purification (Ni-NTA) Immunoblotting, Immunofluorescence Microscopy, Gene Knockout, etc.

 1.4. Manipal Hospital, Bangalore, India.

• Department of Medical Genetics
• Clinical Molecular Geneticist: Clinical Contributions [Jul – Dec 2014]

• Molecular Cytogenetics: FISH and Karyotype for chromosomal analysis of peripheral blood, bone marrow and prenatal sample (amniotic fluid, chorionic villi, and abortus).
• Molecular Genetic Tests: DNA isolation (Blood, CVS, AF, Semen, and Hair), Yq microdeletions, Thalassemia, Maternal cell contamination, SMA Fragile X syndrome and cell-free fetal DNA from the amniotic fluid supernatant.

 1.5. Bir Hospital, Kathmandu, Nepal.

• Department of Pathology
• Medical Laboratory Technologist (OJT): Clinical Contributions [2009  – 2011]

• Clinical Laboratory Analysis: Biochemistry (testing and interpretation of biochemical parameters), Hematology (Testing and interpretation of hematological parameters) Microbiology (identification of specific bacteria and antibiotic sensitivity testing), Serology (testing and interpretation of HIV, HBsAg, HCV, Syphilis, PT, APTT, and clotting factors), Histo/Cytopathology (process biopsy and fluid sample, minor grossing, tissue processing, microtome, and staining) and Parasitology (Semen analysis, Biochemical/Microscopic analysis of urine and stool).

• SRU Centre for Regenerative Medicine and Stem Cell Research, Chennai, India [1^st Feb to 14^th Jun 2014]: 2D and 3D Cell culture (Cardiomyocytes, chondrocytes, neuron, hepatocytes), nanofiber fabrication, cross-linking, cell proliferation assay.
• Centre for Molecular Dynamics Nepal, Intrepid Pvt. Ltd., Kathmandu [2^nd Jul to 17^th Dec 2011]: DNA/RNA isolation, Drug-resistant testing in HIV patients, PCR, RT-PCR, Gel Electrophoresis.

• National Public Health Laboratory, Kathmandu, Nepal [12^th Jul to 11^th Oct 2011]: HIV viral load testing, hormone assay, dengue diagnosis, clinical quality control.
• Anandaban Hospital and Mycobacterial Research Laboratory, Lalitpur, Nepal [21-25 Feb 2011]: Animal handling (Guinea pig), diagnosis of Laprae, PCR, agarose gel electrophoresis, etc.
• B.P. Koirala Memorial Cancer Hospital, Bharatpur, Nepal [1-20 Feb 2011]: Special training in flow cytometry, cryostat sectioning, and immunohistochemistry.
• Nepal Red Cross Society Exhibition Road, Kathmandu, Nepal [12-29 Jan 2011]: Phlebotomist, Blood grouping, cross-matching, separation blood components (plasma, PRP, Platelets, RBC), HIV/HCV/HBV testing. Have good experience of blood collection from a non-remunerated volunteer blood donor.